WebFluorescent protein fusions, though compatible with live cell imaging, substantially increase protein size and can interfere with their biological activity. We demonstrate SR imaging of … WebIn this paper, we provide a general protocol for labeling proteins with the membrane-permeant fluorogenic biarsenical dye fluorescein arsenical hairpin binder-ethanedithiol (FlAsH-EDT₂). Generation of the tetracysteine-tagged protein construct by itself is not described, as this is a protein-specific process.
Short Tetracysteine Tags to β-Tubulin Demonstrate the …
WebFluorescein arsenical helix binder (FlAsH). Source publication Incorporación química de la sonda fluorescente FlAsH en la proteína Hha: aplicación al estudio del complejo Hha/H … dj butzi double t
Protein labelling by reactions between (A) tetracysteine tag and a ...
WebOTHER CATEGORIES OF PRODUCTS. CAST – SKINTAC HX45000. Wrap vinyl Ref : SKINTAC HX45000. CAST – SKINTAC HX30000. Wrap vinyl Ref : HX30CAONEB - SKINTAC HX30000 SATIN CHROME - SKINTAC HX30000 SUPER CHROME - SKINTAC HX30000. CAST – SKINTAC HX20000. Wrap vinyl Ref : SKINTAC HX20000. WebAug 1, 1998 · Tetracysteine (TC) tag labeling has been developed based on the interaction between FlAsH-EDT 2 (fluorescein arsenical helix binder-ethanedithiol) and a 15-amino acid small peptide motif ... WebDec 31, 2008 · A novel affinity matrix based on the bis‐arsenical fluorescein dye FlAsH, which specifically recognizes short α‐helical peptides containing the sequence CCXXCC is designed, and it is found that kinesin tagged with this cysteine‐containing helix binds specifically to FlAs H resin and can be eluted in a fully active form. Genetically‐encoded … becky\\u0027s rag bag