WebApr 17, 2024 · At the end of the differentiation protocol, transcriptional analysis was performed on BRN3A and a marker of pain-sensing nerve cell PRDM12 genes: 1000 times higher for PRDM12 and 2500 times higher for BRN3A in differentiated cells than they were in undifferentiated SKPs. Using immunostaining, we showed that 65% and 80% of cells … WebNov 29, 2024 · Mechanistically, ER–mitochondria coupling was increased through activation of the IP3R1–GRP75–VDAC1 axis, which transferred Ca2+ from the ER to the mitochondria. Elevated mitochondrial Ca2+ led to an increase in mitochondrial ROS and a decline in mitochondrial membrane potential.
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Brn-3b : a POU domain gene expressed in a subset of
WebThe cells were incubated for 1 hr at room temperature with a mouse anti-rat primary antibody against CD90/Thy 1.1 (1:200; ab225; Abcam, Toronto, Ontario, Canada) followed by a goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 488 (1:1000; Life Technologies Inc., Burlington, Ontario, Canada) at room temperature in the dark for 1 hr. WebAbcam - antibodies and reagents supplier, find any antibody WebView specific protocols for BRN3A Antibody (NB110-57427): Immunohistochemistry Protocol for BRN3A Antibody (NB110-57427) Find general support by application which … hawkeye cyber security